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KMID : 0381920110410030169
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Korean Journal of Microscopy
2011 Volume.41 No. 3 p.169 ~ p.177
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Inhibitory Efficacy of Black Tea Water Extract on Melanogenesis in Melan-a Cells and Its Action Mechanisms
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Choi So-Young
Kim Young-Chul
Chang Byung-Soo
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Abstract
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To evaluate the whitening effect of Camellia sinensis water extract (CSWE), CSWE was treated to melan-a cells. Total polyphenol contents and flavonoid contents of CSWE were 102 mg/g and 87 mg/g, respectively. The electron-donating ability of CSWE revealed a dose-dependent response, showing the excellent ability of 82% at 800 μg/mL, and which was higher than the arbutin (48%). The CSWE significantly (p?0.00l) suppressed the melanin synthesis and the development of melanocyte dendrites was inhibited in a dose-dependent manner. The CSWE significantly (p?0.00l) inhibited both intra-cellular and cell-extracted tyrosinase activities. And inhibitory efficacies of CSWE on both melanin synthesis and tyrosinase activity were significantly (p?0.00l) higher than the arbutin. The tyrosinase protein expression was not influenced by arbutin treatment. However, CSWE treatment significantly (p?0.00l) reduced it. Both arbutin and CSWE treatment did not influence on mRNA expressions of tyrosinase, tyrosinase-related protein-1 and tyrosinase related
protein-2.
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KEYWORD
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Black tea, Melan-a cell, Tyrosinase, Whitening
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